Our study showed that in vitro treatment of various breast cancer and melanoma cell lines induced several stress-related molecular processes characteristic of immunogenic cell death: The induction of p-eIF2α indicated the triggering of an ER stress response and we proved increased mitochondrial ROS production, calreticulin exposure on the surface of pre-apoptotic cells and cell surface expression or release of the DAMPs calreticulin, hsp70, hsp90 and ATP. This evidence concerns the gene CALR and breast cancer.