LV-Control, LV-USP1, and wild-type (WT) cells were infected with four subtypes of influenza strains (H1 N1, H3 N2, BY, BV) at MOI = 0.01, and the supernatant samples were collected at 12 h, 24 h, 36 h, 48 h, and 60 h post-infection for the TCID50 assay. The gene discussed is USP1; the disease is infection.