Subsequently, 9 genes associated with lipid (PLIN1, SERPINE1, LPL, FABP4, CYP7A1, NOS2, CDKN1A, PRKCE, and CXCL9) and 10 genes related to the immune system (IL32, IL20RB, PRKCE, NOS2, RAB7B, CXCL9, ACKR3, LIF, SERPINE1, and CDKN1A) were identified in NAFLD vs. control group. This evidence concerns the gene NOS2 and metabolic dysfunction-associated steatotic liver disease.