Additionally, the top 5 biological functional hub genes in NAFLD vs. control (CXCL9, NOS2, SERPINE1, FABP4, and LPL) and NAFLD vs. cirrhosis (AKR1D1, UGT2B17, CYP26B1, LIPC, and DGAT2) groups were identified through PPI analysis among lipid, immune, and metabolism dysregulated genes. This evidence concerns the gene SERPINE1 and metabolic dysfunction-associated steatotic liver disease.