Additionally, the top 5 biological functional hub genes in NAFLD vs. control (CXCL9, NOS2, SERPINE1, FABP4, and LPL) and NAFLD vs. cirrhosis (AKR1D1, UGT2B17, CYP26B1, LIPC, and DGAT2) groups were identified through PPI analysis among lipid, immune, and metabolism dysregulated genes. The gene discussed is NOS2; the disease is metabolic dysfunction-associated steatotic liver disease.