CD8A and neoplasm: As we expected, the TCR trajectory showed that most of the tumor-specific CD8+ T cells were expanded from the memory T cells in mediastinal lymph nodes (Figure 5E). The scatter plot also demonstrated that tumor-specific CD8+ T cells that highly expanded in primary tumor sites mostly shared TCR clones of memory T cells (dual expanded clonotypes) in mediastinal lymph nodes (Figure 5F).