GPT and Abnormal renal physiology: As shown in Figure 6A–I, serum biochemical assays showed mild effects of 2K2C in each group at 1–2 weeks, but 2K2C model prepared by 0.2 mm silver clips showed increased CK, LDH, ALT, AST, urea levels (Figure 6A–E), and decreased serum K+ level at 4–6 weeks after 2K2C surgery (Figure 6I), indicating myocardial and hepatic injury and renal dysfunction, whereas 0.3 mm and 0.25 mm clip groups were still less affected in the biochemical indicators.