Overexpression of GFP affects a variety of genes related to the metabolism and synthesis of amino acids, whereas intracellular gene changes in HEK293T cells induced by overexpression of ADORA3 are more enriched for a variety of malignant tumors and neurodegenerative disorders, which provides valuable insights for subsequent studies of GFP and ADORA3. This evidence concerns the gene ADORA3 and cancer.