The IRS and positive rate of ERα in the stroma and epithelium of normal, CIN2/3, CIS, and ICC specimens were 0.9 ± 1.1 (51.7%) vs. 0.5 ± 0.7 (30.9%), 1.3 ± 1.5 (65.1%) vs. 0.8 ± 1.2 (35.3%), 2.1 ± 1.9 (81.4%) vs. 1.1 ± 1.4 (61.4%), and 3.6 ± 3.3 (82.8%) vs. 0.8 ± 1.7 (28.6%), respectively (Table 1). This evidence concerns the gene ESR1 and intrahepatic cholangiocarcinoma.