TLR3 and osteosarcoma: Transcriptomic data support this observation because actinomycin D and nutlin-3a acting alone and in combination up-regulate TLR3 expression in the A549 cell line, and A+N up-regulates TLR3 expression in melanoma (A375), osteosarcoma (U-2 OS), and another lung cancer (NCI-H460) cell line (Table 1) [5].