These data suggested that overexpressed alternative splicing of LDLR pre-mRNA could occur in healthy young adults with normal BMI prior to the appearance of hypercholesteremia and/or obesity, indicating augmented levels of LDLR-ΔExon4 and ΔExon12 leading to impaired cholesterol metabolism. This evidence concerns the gene LDLR and obesity due to melanocortin 4 receptor deficiency.