To better understand the mechanism leading to SLIT2/SLIT3 expression, and subsequently miR-218-5p suppression in CRC, DNA samples from five different CRC cell lines (SW-480, DLD-1, LoVo, HT-29, and HCT116) and one non-cancerous epithelial cell line, MCF10A, were used as input for methylation analysis using the bisulfite method. Here, SLIT3 is linked to colorectal carcinoma.