The distribution of methylation along the reference genomes on chromosomes four (chr4:169834935-169838614) and five (chr5:169836058–169837692) for the promoter regions of SLIT2 and SLIT3, respectively, showed substantial hypermethylation along the entire promoter regions in CRC, in comparison with MCF10A cells (Figure 7D). This evidence concerns the gene SLIT2 and colorectal carcinoma.