The antiproliferative activity of compound 1C was assessed using the methylthiazoltetrazolium (MTT) colorimetric assay in cancerous (parental human colorectal adenocarcinoma cells, COLO 205, and human colorectal adenocarcinoma cells overexpressing ABCB1, COLO 320) and non-cancerous (human foreskin fibroblasts, BJ-5ta) cell lines, with the cells being treated with increasing concentrations of the compound. Here, ABCB1 is linked to colorectal adenocarcinoma.