Based on these premises, in the present study, we investigated whether the Glo1/MG-H1 axis was involved in ACh’s protumorigenic role through OPN control in two human PCa cell lines, LNCaP and PC3, representing extensively studied cell models of poorly aggressive [16,17] and bone metastasis-derived PCa [16,17], respectively. This evidence concerns the gene SPP1 and posterior cortical atrophy.