This is the first study analyzing simultaneously the expression pattern of TLR4, TLR5 and TLR9 in human gastric biopsies from paired cases collected in two independent visits separated by 3 years, and correlating with the H. pylori status and histological assessment of atrophy and intestinal metaplasia by Operative Link for Gastritis Assessment (OLGA) and Operative Link on Gastric Intestinal Metaplasia (OLGIM) scoring. This evidence concerns the gene TLR5 and gastritis.