To study the anti-tumor activity of huCC49-IL-2 in an immunocompetent model, we generated murine carcinoma cell lines expressing the target antigen TAG-72 via transduction with an epHIV7 lentivirus carrying the murine st6galnac-I gene (mSTn) under the control of the EF1α promoter, as described by Lee et al. [4]. Here, MSTN is linked to carcinoma.