This study aims to investigate FTO and ALKBH5 in an RCC cohort from the MENA region using targeted DNA sequencing to screen for FTO and ALKBH5 variants, as well as whole transcriptomic analysis and immunohistochemistry (IHC) to identify the expression patterns of FTO and ALKBH5 associated with RCC. The gene discussed is ALKBH5; the disease is renal cell carcinoma.