Finally, scRNA-seq and ST-identified neutrophil marker genes were combined with TCGA-HNSC transcriptome data to perform WGCNA and ssGSEA analyses, and biomarkers MX2 and HCK of N1-TANs which may be associated with the MTC efficacy in LSCC were identified. This evidence concerns the gene MX2 and medullary thyroid gland carcinoma.