Consistent with the results of the coculture experiments, the results of immunofluorescence staining and Western blotting revealed that the levels of pyroptosis-related markers, such as cleaved caspase-1, N-terminal GSDMD, and cleaved IL-1β, were significantly increased in D-BCC-EXOs but not in N-BCC-EXOs (Fig. 2i, j and m). The gene discussed is CASP1; the disease is skin basal cell carcinoma.