The aim of study was to quantify and compare the methylation levels of MLH1, MGMT, p16INK4a, and LINE-1, and the gene expression levels of MLH1, MGMT, and p16INK4a in colon tumors, matched non-cancerous mucosa and control mucosa to identify early signs of putatively premalignant changes. This evidence concerns the gene MLH1 and colonic neoplasm.