Analysis of fibroblasts by enrichment analysis, KEGG analysis of single-cell data showed that fibroblast-associated functions (collagen binding, growth factor binding, fibronectin binding, IL-17 signaling pathway) and CXCL related pathways (PI3K-Akt signaling pathway, IL-17 signaling pathway) were significantly enriched in fibroblasts from metastatic tumors (Figure 1G). This evidence concerns the gene AKT1 and metastatic neoplasm.