In this study, Akt, mTOR, and Pax-2 were detected in ECT smear and paraffin tissue sections for each enrolled subject, and the dataset outcomes demonstrated that Akt, mTOR, and Pax-2 expressions were highly associated (p < 0.001), indicating that ICC method in ECT was stable and reliable. This evidence concerns the gene AKT1 and intrahepatic cholangiocarcinoma.