A systematic analysis of randomized controlled trials involving overweight/obese Iranian females (aged 15-48 years) demonstrated that probiotic and Prebiotics supplementation significantly ameliorates metabolic and endocrine abnormalities: Insulin sensitivity: Marked reductions in Homeostasis Model Assessment of Insulin Resistance (HOMA-IR) index, FBG, and fasting insulin levels were observed (all P<0.05). The gene discussed is INS; the disease is Insulin resistance.