BDNF and major depressive disorder: Similarly, Figure 8A shows that pre-treatment with BDNF-shRNA significantly abolished the antidepressant-like effects of chiglitazar in the CUMS model of depression, as the (CUMS + chiglitazar + BDNF-shRNA)-treated mice displayed significantly higher immobility in the FST [ANOVA: F(6, 63) = 23.941, P < 0.01] and TST [ANOVA: F(6, 63) = 27.445, P < 0.01] as well as lower sucrose preference [ANOVA: F(6, 63) = 19.301, P < 0.01] than both the (CUMS + chiglitazar)-treated and (CUMS + chiglitazar + Control-shRNA)-treated mice (n = 10, P < 0.01).