A third study specifically assessed PAM50-determined intrinsic subtypes in matched FFPE and frozen primary early breast cancer specimens from 94 patients, and reported an overall discordance rate of 20% (19/94), including 27% (15/54) of LumA FFPE, 6% (1/18) of LumB FFPE, and 33% (3/9) of HER2-E FFPE specimens being discordant with the matched frozen subtype call [46]. The gene discussed is TMEM43; the disease is breast carcinoma.