To confirm that the effect of QRICH1 promoting cardiac hypertrophy in vivo is due to the activation of ATF6 in cardiomyocytes under growth stimuli, we used dual AAV9 injections to evaluate the effects of ATF6 overexpression on cardiac phenotypes in QRICH1 KD mice (Supplementary Fig. 9A-C). The gene discussed is ATF6; the disease is cardiac hypertrophy.