INS and type 2 diabetes mellitus: Both G3BP1−/− and G3BP2−/− cells showed impaired glucose-stimulated insulin secretion with a reduced stimulation index compared to WT MIN6-K8 cells (Fig. EV5E,G), with G3BP1−/− cells displaying also a higher proinsulin release in resting conditions (Fig. EV5D), which is a hallmark of beta cell dysfunction in type 2 diabetes.