In addition, quantitative morphometric 3D analysis of microglia revealed that the absence of gut microbiota, both in WT and AppNL-G-F AD mice, resulted in longer processes and increased numbers of segments, branching, terminal points, and volume of IBA1+ microglia, typical for a more ramified microglial morphology (Figure 2(c,d)). This evidence concerns the gene AIF1 and Alzheimer disease.