Taking patient age, prostate volume, serum total PSA, and the performances of the four ultrasonic methods for diagnosing prostate nodules as independent variables and the pathological results as the dependent variable, the results of binomial logistic regression analysis showed that enhancement strength on CEUS, time to enhancement on CEUS, presence of a blue area on TRTE and total serum PSA were statistically significant independent factors for diagnosing PCa. Here, KLK3 is linked to posterior cortical atrophy.