To investigate whether KLF4 induces T‐cell differentiation in T‐ALL cell lines, we lentivirally transduced a Doxycycline (Dox)‐inducible KLF4 expression vector into six T‐ALL cell lines (Jurkat, MOLT‐3, CCRF‐CEM, RPMI‐8402, HPB‐ALL, and TALL‐1), establishing Dox‐inducible (Di)‐KLF4 T‐ALL cell lines. Here, KLF4 is linked to acute lymphoblastic leukemia.