In consistent with the fungal burden analysis, the pathogenicity of rtf1Δ mutant in the intravenous model of cryptococcosis were significantly attenuated compared to the wild-type strain and strains complemented with the full length of Rtf1 or the HMD domain, while the Plus3, Rtf1E95A, or HMD E95A failed to complement the attenuated virulence of the rtf1Δ mutant (Figure 5G). The gene discussed is RTF1; the disease is cryptococcosis.