In summary, the in vivo knockdown of Hba-a1 and S100a8 prolonged the survival of HCC-bearing mice already after one T cell transfer, thereby decreasing liver biochemical parameters, CD19+ IL-10+ B cells, PD-L1-expressing DCs, and macrophages as well as Tregs and inhibitory markers, like PD-1, CD160 and 4-1BBL, on T cells. This evidence concerns the gene CD274 and hepatocellular carcinoma.