Importantly, Tregs counts (CD4+ GFP+ Foxp3+/CD4+ GFP+ eFluor450+ Foxp3+) remained low and well controlled in the shHba-a1 and shS100a8 groups, starting from day 44 until HCC development, in contrast to the shRen control group (Supplementary Figure S17A). Here, CD4 is linked to hepatocellular carcinoma.