Importantly, among the enriched shRNAs in the pooled analysis of both, CD4 and CD8 T cell populations, several shRNAs were present and targeted prominent genes, such as: CD200 receptor 1 (CD200r1), calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacnad1), and translocator protein (Tspo), which were found in T cells isolated from liver and/or relLN, the local organs of HCC development (Figures 3, 4). This evidence concerns the gene TSPO and hepatocellular carcinoma.