In addition, the GSEA analysis in this study showed the depression of multiple cancer-promoting pathways, such as the TGF-β signaling pathway, hypoxia, EMT, glycolysis, AND angiogenesis, as well as K-RAS signaling pathways, implying that FOXN4 exerts a tumor-suppressive effect in LUAD by repressing these pathways. Here, TGFB1 is linked to cancer.