EVs subpopulations such as CD63+ EphA+ EVs were previously described to be significantly over expressed in PC cell-lines compared to normal pancreatic cell line as well as in PC patient serum, with high diagnostic potential when combined with the traditional CA19.9 (AUC 0.958, P 1⁄4 0.0007) (48). The gene discussed is CD63; the disease is pachyonychia congenita.