CD40LG and primary biliary cholangitis: PBC is diagnosed based on the biochemical criteria of raised serum alkaline phosphatase (ALP), liver histology showing cholangitis and destructive interlobular bile ducts, elevated IgM, raised aminotransferases, and the presence of highly disease-specific autoantibodies like AMA and anti-nuclear antibodies (ANA) (Sp100 and anti-glycoprotein-gp210) [19,61,62].