From the second training day onward, the Model group demonstrated significantly prolonged escape latencies (Figure 4B, Day 2: **P < 0.01, Day 3: ****P < 0.0001, Day 4: **P < 0.01, Day 5: ***P < 0.001 vs Control group), increased swimming distances (Figure 4C, ****P < 0.0001 vs Control group, η2 = 0.797), and fewer platform crossings (Figure 4E, ****P < 0.0001 vs Control group, η2 = 0.883), indicating cognitive deficits in APP/PS1 mice. This evidence concerns the gene APP and Cognitive impairment.