PDCD1 and non-small cell lung carcinoma: We analyzed publicly available scRNA-seq datasets of CD8+ T cells from tumors and JTs from patients with CRC20 and NSCLC.25 We observed significantly higher PDCD1 gene expression in CD8+ T cells from tumors compared to JTs in both CRC and NSCLC patients (Figure 6a and Supplementary Figure S6a).