Detection of the level of mIL-10 in tumor tissues showed that at 8 hours after treatment, the expression level of mIL-10 in the CAL-101 combined with VVL-TD-mIL-27 group tended to increase compared with the CAL-101 combined VVL-TD-RFP group, and at 72 hours after treatment the mIL-10 expression level in this group was significantly increased (online supplemental figure S6Fi,ii), indicating that IL-27 induced IL-10 production, which is consistent with previous results.33 The gene discussed is IL10; the disease is neoplasm.