Oral cancer cell (SCC070) exposed to CM collected from TGF-CAF transfected with Tie2-siRNA (TGF > > siTie2-CAF) showed significant downregulation of tested stemness related genes NANOG, OCT4, ALDH1 A1, CK14 and CD44 (Fig. 7D) as well as spheroid forming efficiency, as compared to CM collected from CAF transfected with control-siRNA (TGF > > siControl-CAF) (Fig. 7D, i-ii). This evidence concerns the gene NANOG and lip and oral cavity carcinoma.