In order to generate an organoid culture, we isolated ApcΔ/ΔSmad4Δ/Δ (Smad4Δ/Δ) and ApcΔ/ΔSmad4+/+ (Smad4+/+) caecal adenoma and generated in vitro cultures in Matrigel using published conditions24,29. This evidence concerns the gene SMAD4 and adenoma.