Six CD8+ bulk cell lines specific to either ILY, ILD, or SLS were then individually enriched by tetramers, and the functionality of these antigen-specific T cells was evaluated by intracellular cytokine staining (ICS) after co-culturing T cells with VACV-infected EBV-transformed B cell lines (BCLs) (Fig. 3D). Here, CD8A is linked to interstitial lung disease.