To investigate if differences in intratumoral CD4+ T cell infiltration were based on a differential immune activation pattern in the peripheral blood, we performed flow cytometry analyses on peripheral blood from patients with NSCLC BM and compared the T cell immunophenotypes to those of age-matched healthy individuals using five different multicolor antibody staining cocktails (Fig. 2a). The gene discussed is CD4; the disease is non-small cell lung carcinoma.