To explore the role of eIF3a in the MCT-induced PAH rat model, the rats were subjected to intratracheal injection of AAV1-carried scrambled shRNA (AAV1-shRNA-NC) as a negative control or AAV1-carried eIF3a shRNA (AAV-shRNA-eIF3a) to knock down the expression of eIF3a. Here, EIF3A is linked to pulmonary arterial hypertension.