Glucose uptake measurements in differentiated myotubes can reflect insulin sensitivity (Chanon et al. 2017) and insulin resistance can be induced in cultured muscle cells from both non-diabetic and diabetic subjects through hyperinsulinemia, accompanied by increased GLUT1 but unaltered GLUT4 levels (Ciaraldi et al. 1995). This evidence concerns the gene INS and Hyperinsulinemia.