CD8A and neoplasm: To distinguish whether disruption of MLKL-mediated tumor cell necroptosis only affected cross-priming of CD8+ T cells or whether it intrinsically impaired tumor cell susceptibility to cytolytic T cell-mediated killing, we established a model in which mice were inoculated with a wild-type tumor in one flank and a MLKL−/− tumor in the other flank (figure 3H).