Notably, secreted TIMP-1glyc 1/1 harboring both glycans at N30 and N78 was more potent than the TIMP-1glyc 0/1 variant harboring the glycan at N78 alone, demonstrating that the occupation of the N30 glycosylation site, as observed in PC patient plasma (Fig. 1), is decisive for TIMP-1 to exhibit its full tumor cell-promoting cytokine potential. The gene discussed is TIMP1; the disease is pachyonychia congenita.