We observed that the ∼29 kDa band corresponded to double-glycosylated TIMP-1 (dgTIMP-1), the ∼25 kDa band to monoglycosylated TIMP-1, and the ∼20 kDa band to nonglycosylated TIMP-1, revealing a patient-specific distinct pattern of a majority of dgTIMP-1 in PC patients as compared to a rather equal distribution of all variants in healthy donors. Here, TIMP1 is linked to pachyonychia congenita.