The integration of single‐cell RNA sequencing, whole genome sequencing, and exome sequencing,[52] as well as multiplex single‐cell RNA sequencing and multiplex single‐cell MALBAC genome sequencing, could identified evidence of gene mutations in the CAFs of CRC and found that BGN, RCN3, TAGLN, MYL9, and TPM2 mutations are prevalent in CRC, which plays a crucial role in the pathogenesis and progression of CRC.[53] However, these reports did not specify the presence of PIK3CAH1047R mutation, and the origin and function of these mutations in CAFs remain unclear. The gene discussed is RCN3; the disease is colorectal carcinoma.