Similar to FMT and LCA diet mouse MASLD models, Gpbar1 knockdown in hepatocytes noticeably inhibited MASLD progression in both models, as manifested as reduced hepatocyte vacuolization by H&E staining, decreased Oil Red O‐stained cells, attenuated hepatic fibrosis by Masson's trichrome staining, and alleviated hepatic inflammation by F4/80 staining, compared to Gpbar1fl/fl mice (Figure 2D and Figure S3B, Supporting Information). Here, GPBAR1 is linked to Leber congenital amaurosis.