To date, the best-characterized negative regulator of PINK1/Parkin-induced mitophagy signaling is the deubiquitinase (DUB) USP30, discovered following an overexpression screen with a Flag-tagged human DUB cDNA library, with phase 1 human trials of USP30 inhibitors underway in patients with PD (Mission Therapeutics) (48). This evidence concerns the gene ZUP1 and Parkinson disease.