To overcome this impediment, we used an F1 hybrid approach whereby genetically diverse inbred CC strains were crossed to the inbred genetic background of the transplanted tumor cell line (Figure 1A).33 We chose multiple murine tumor cell lines from two genetic backgrounds, C57BL/6J (B6) and BALB/cJ (BALB), covering both mammary gland and colon cancers: MC38 (colon; B6), CT26 (colon; BALB), AT3 (mammary gland; B6), and EMT6 (mammary gland; BALB). This evidence concerns the gene DDX53 and malignant colon neoplasm.