Furthermore, since chronic hyperinsulinemia is observed in people with obesity and metabolic disorders (52, 53), further investigations are needed to evaluate whether prolonged exposure to a physiological concentration of insulin induces sensitization to mechanical stimulation in DRG neurons by enhancing sensitization of TRPV4 channels as observed in this study. The gene discussed is TRPV4; the disease is obesity due to melanocortin 4 receptor deficiency.