Mediation analysis revealed that levels of 7-alpha-hydroxy-3-oxo-4-cholestenoate (7-hoca) (MP = − 14.6%) and Palmitoyl dihydrosphingomyelin (d18:0/16:0) (MP = 7.9%) partially mediated the relationship between CD3 on CD39( +) resting Treg cells and glioma. This evidence concerns the gene ENTPD1 and central nervous system cancer.